Scanner Microarray InnoScan 710-IR

“Able to read signals in the infrared spectrum”

InnoScan 710 Infrared Microarray Scanner
The Innoscan 710-IR is able to read signals in the near infrared spectrum. This allows a considerable reduction of the background noise caused by the supports normally used in protein microarrays such as nitrocellulose or PVDF.

Main features

• Increased sensitivity of protein microarrays thanks to infrared fluorescence detection: with its two excitation sources in the near infrared spectrum (670nm and 785nm), it is possible to read slides with nitrocellulose or PVDF substrates without the problem of autofluorescence, caused by these supports. This results in increased test sensitivity by significantly increasing the signal-to-noise ratio.
• Real-time autofocus optimized for nitrocellulose or PVDF substrates: The Innoscan 710-IR scanner uses a real-time autofocus system that produces perfect homogeneity across the entire reading surface. A special configuration made for the program, Mapix, allows users to adapt the autofocus system to nitrocellulose or PVDF formats.
• Speed: With an adjustable reading speed from 10 to 35 lines per second, users can get test results very quickly (4 minutes to read an entire slide at 10 µm / pixel resolutions).

Application

The Innoscan 710-IR is ideal for reading slides on nitrocellulose or PVDF support, or any slide stained with fluorophores excited at near infrared wavelengths (670nm, 785nm):

• Protein microarray
• ELISA arrays
• RPPA
• Enzyme microarray

Fields of application:

• Proteomics: detection of high-yield protein expression.
• Studies on cellular signals: profiling of proteins and protein activity linked to cellular signaling pathways.

Technology:

The Innoscan 710-IR is a confocal fluorescence scanner equipped with two excitation lasers (670nm and 785nm) and two photomultiplier tubes (PMT) as a detection system. The PMT detection system is extremely sensitive and perfectly linear over the entire range of functions (from 0 to 100%) allowing users to adjust the PMT gain of each wavelength independently of the other.

Since the Innoscan 710-IR contains a real-time autofocus system, users have the advantage of eliminating the effect of substrate deformation, thus allowing perfect homogeneity across the entire reading surface.

Innopsys’ Innoscan scanners are known for their excellent photometric properties, particularly in terms of sensitivity and signal-to-noise ratio (SNR).

Thanks to the XDR (Extended Dynamic Range) mode, Innoscan scanners have a dynamic range greater than 106 and are therefore able to detect the smallest points while avoiding saturated ones.

Brochure