Scanner Microarray InnoScan 710-IR

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Scanner Microarray InnoScan 710 Two-color
20 Ottobre 2016
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Scanner Microarray InnoScan 910 High Density
20 Ottobre 2016

Scanner Microarray InnoScan 710-IR

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Scanner Microarray InnoScan 710-IR Infrared detection dedicated for protein arrays, Innopsys by Galli

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“ Capable of reading signals
in the near-Infrared spectrum ”

InnoScan 710
Scanner per Microarray ad Infrarosso

The Innoscan 710-IR is capable of reading signals in the near-Infrared spectrum. This allows for a considerable reduction in the background noise caused by supports normally used in protein microarrays such as nitrocellulose or PVDF.

Main Features

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Scanner MicroArray Innoscan 710-IR InfraRed

  • Increased sensitivity of protein microarrays thanks to Infrared fluorescence detection: with its two sources of excitation in the near infrared spectrum (670 nm and 785nm), it is possible to read slides with nitrocellulose or PVDF substrates without the problem of auto-fluorescence caused by these supports. This translates to an increased sensitivity of tests by considerably increasing the signal/background noise ratio.
  • Real-time autofocus which is optimized for nitrocellulose or PVDF substrates: The Innoscan 710-IR scanner uses a real-time autofocus system which produces a perfect homogeneity across the entire reading surface. A special configuration made for the program, Mapix, allows users to adapt the autofocus system to nitrocellulose or PVDF formats.
  • Speed: With an adjustable reading speed of 10 to 35 lines per second, users can obtain test results very quickly (4 minutes to read an entire slide with resolutions of 10 µm/pixel).

Applications

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The Innoscan 710-IR is ideal for reading slides on a nitrocellulose or PVDF support, or any slide dyed with fluorophores excited at near infrared wavelengths (670nm, 785nm):

  • Protein microarrays
  • ELISA arrays
  • RPPA
  • Enzymatic Microarrays

Application Fields :

  • Proteomic : High-throughput protein expression detection.
  • Cell signal studies: profiling of proteins and protein activity tied to cellular signal pathways.

Technology

The Innoscan 710-IR is a confocal fluorescence scanner equipped with two excitation lasers (670nm and 785nm) and two photomultiplier tubes (PMT) as a detection system. The PMT detection system is extremely sensitive and perfectly linear across the entire function range (0 to 100%) thus allowing users to adjust the PMT gain of each wavelength independent of the other.

As the Innoscan 710-IR contains a real-time autofocus system, users have the advantage of eliminating the effect of substrate deformation, thus allowing a perfect homogeneity across the entire reading surface. Innopsys’ InnoScan scanners are known for their excellent photometric properties, notably in terms ofsensitivity and signal to noise ratio (SNR).

Thanks to XDR mode (Extended Dynamic Range), InnoScan scanners have a dynamic range over 106 and then are capable to detect the smallest spots avoiding saturated spots

Specific Techniques

Spécifications Data
Resolution 3 – 40 µm / pixel
Excition Wavelengths 670 nm and 785 nm
Compatible Fluorophores 670 nm : AlexaFluor 700, Dylight 680, IRDye 680, AlexaFluor 680

785 nm : AlexaFluor 790, IRDye 800, Dy-light 800

Compatible Slides Microscope slides of the standard norm ISO 8037-1

25-26 x 75-76 mm2 / 1” x 3” ; thickness: 0.9 – 1.2 mm

Maximum Reading Zone 22 x 74 mm²
Focus Automatic or manual
Detection Digital PMT and real confocal detection
PMT Gain Linear from 0 to 100%
Reading Modes Simultaneous or sequential
Reading Speed 10 to 35 lines/sec
Read Time 3,55 minutes for a full slide scan at a resolution of 10 µm / pixel
Dimensions 12 x 18 x 16 in
Weight 15,5 Kg (35 lbs)
Interface Ethernet
Bar Code Reader Yes
Image Analysis Program Mapix
Image Format 16 and 20 bit TIFF

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